Figure 2.
Production of interleukin (IL)-6 (a and c) and tumour necrosis factor (TNF)-α (b and d) by in vitro L2-mouse hepatitis virus 3 (MHV3)-infected C57BL/6 peritoneal macrophages in the presence of an anti-carcinoembryonic cell adhesion antigen 1a (CEACAM1a) antibody or a SH2 domain-containing protein (SHP)-1 inhibitor. Cells were treated or not with a monoclonal anti-CEACAM1a monoclonal antibody (mAb) (AgB10 at 2 μg/106 cells) 2 hr before infection (a and b) or with a SHP-1 inhibitor [sodium stibogluconate (SS) at 10 μm] 15 min before infection (c and d) and infected further with 0·1–1·0 multiplicity of infection (MOI) of L2-MHV3 for 24 hr. The IL-6 and TNF-α levels were evaluated by enzyme-linked immunosorbent assay (ELISA). Results are representative of three experiments (***P < 0·001).