Table 1.
Peripheral blood CD27+ IgG+ B cells show the highest proliferation and immunoglobulin G secretion rate
| Short term2 |
Long term3 |
||||
|---|---|---|---|---|---|
| Phenotype1 | tgen (hr) | BrdU (OD 450 nm) | IgG ng/ml | Expansion (fold) | IgG ng/106 cells/hr |
| CD19+ | 61 ± 3 | 1·35 ± 0·06 | 1114 ± 100 | 151 ± 9 | 1519 ± 65 |
| CD27− IgG− | 72 ± 5 | 1·34 ± 0·18 | 14 ± 1 | 46 ± 1 | 906 ± 68 |
| CD27+ IgG+ | 33 ± 2 | 1·84 ± 0·07 | 21548 ± 1044 | 300 ± 18 | 2126 ± 3 |
tgen, generation time; IgG, immunoglobulin G; BrdU, 5-bromo-2′-deoxy-uridine.
1
Total CD19+ and sorted CD19+ CD27− IgG− and CD19+ CD27+IgG+ B cells were stimulated with CD154 in the presence of interleukin-2 (IL-2), IL-4 and IL-10. These results are representative of two independent experiments. SD represented variations among triplicate values obtained in this particular experiment.
2
tgen and total IgG accumulations were determined over the first 5 days, and BrdU incorporation was measured from day 4 to 5. Viability of all the cells remained > 93% (data not shown).
3
Expansion and IgG secretion were determined on day 13.