Figure 1.

 EH-mediated binding to peptides and proteins in vitro and in vivo. (A) Schematic of the Eps15 and Eps15R proteins with their EH domains. Amino acids positions are indicated. (B) Predicted amino acid sequence of peptides selected by screening of a random phage-displayed peptide library with GST–EH and GST–EHR, from Eps15 and Eps15R, respectively. NPFs are in boldface type. (C) NPF-containing motifs present in the cDNAs identified by screening of a human fibroblast expression library using GST–EH as a probe. Underlined peptides were used for the in vitro binding experiments described in Figs. 4, 6, and 8.