Figure 2.

 Human NUMB and NUMB-R cDNAs and proteins. (A) The structures of the human NUMB (hNUMB) and NUMB-R (hNUMB-R) cDNAs are depicted. The ORFs are labeled. Positions are indicated in kb. The nucleotide positions of initiator and terminator codons are also indicated. Canonical polyadenylation sites (AATAAA) are at positions 2649, 2823, 2958, and 3025, for hNUMB and hNUMB-R, respectively (not shown). (B) Predicted protein sequences and alignment of human NUMB and NUMB-R. In the hNUMB-R sequence, only nonidentical amino acids are reported, except for the NPF motifs. Dashes indicate gaps introduced to maximize the alignment. Accepted conservations, employed to calculate relatedness, are D, E, N, Q; L, I, V, M; K, R, H; F, Y, W; and A, G, P, S, T. The PTB domains and the NPF motifs are indicated in reverse print.