Figure 5.
Effect of indoleamine 2,3-dioxygenase (IDO) on granulocyte colony-stimulating factor (G-CSF)-induced immature myeloid cell (gMC)-mediated inhibition of acute graft-versus-host disease (GVHD). (a) B6D2F1-recipient mice were lethally irradiated (950 cGy) and transferred with 2 × 106 B6 donor T cells alone or plus 2 × 106 gMC. Recipient mice were orally administered with 1-methyl-dl-tryptophan (1-MT) or control vehicle daily from day − 1 until 4 days after the cells were transferred. (b) The gMCs were isolated from G-CSF-injected wild-type (WT) and IDO−/− mice and cotransferred with donor T cells to irradiated recipient mice. Each group consisted of eight mice. Data represent the results from two similar experiments.