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. 2009 Sep;128(1 Pt 2):e296–e305. doi: 10.1111/j.1365-2567.2008.02962.x

Figure 3.

Figure 3

Cytokine messenger RNA (mRNA) expression in guinea pig spleen cells. Total spleen cells from non-vaccinated (NV) and bacillus Calmette–Guérin-vaccinated (BCG) guinea pigs 5 weeks following Mycobacterium tuberculosis infection were stimulated with concanavalin A (Con A), lipopolysaccharide (LPS) or purified protein derivative (PPD) for 24 hr as described above. Interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), interleukin-10 (IL-10) and IL-12p40 mRNA expression was quantified using real-time reverse transcription–polymerase chain reaction. Fold induction of mRNA was calculated as described in Fig. 2. The results are expressed as mean and the standard errors of means from five animals. The differences in the fold induction between the cultures from non-vaccinated and BCG-vaccinated groups were determined by anova (‡P< −0·05).