Figure 3.

Memory subset composition of ex vivo cervical cytobrush-derived T cells. (a) Representative plots of staining combinations used to differentiate memory subsets of cervical T cells based on differential expression of CD3, CD8, CD45RA, CCR7 and CD27. (b) CD45RA⁺ CD27⁺ CCR7⁺ (naïve), CD45RA⁺ CD27⁻ CCR7⁺, CD45RA⁺ CD27⁺ CCR7⁻, CD45RA⁺ CD27⁻ CCR7⁻ (effector memory cells expressing RA (EMRA)), CD45RA⁻ CD27⁺ CCR7⁻, CD45RA⁻ CD27⁺ CCR7⁺ (central memory), CD45RA⁻ CD27⁻ CCR7⁺ and CD45RA⁻ CD27⁻ CCR7⁻ (effector memory) CD8⁺ (left panel) and CD8⁻ (right panel) T-cell frequencies in cervical cytobrush samples derived from HIV-infected (black bars) and uninfected (clear bars) women. Each bar represents the mean percentage memory marker expression by CD8⁻ and CD8⁺ T cells (± standard deviation). The unpaired Student’s t-test was applied to compare cervical counts in HIV-negative versus HIV-positive women. P-values ≤0·05 were considered significant.