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. 2009 Sep;128(1 Pt 2):e805–e815. doi: 10.1111/j.1365-2567.2009.03086.x

Figure 4.

Figure 4

Attenuated inflammatory response by cells exposed to bacteria for 7 days. Bone marrow cells were cultured for 7 days in granulocyte–macrophage colony-stimulating factor and exposed to bacteria (1 : 1 ratio) at D0, D3 or D5 of 7-day culture. On day 7, cells were harvested and washed and were either re-plated in complete media or in media with lipopolysaccharide (LPS; 1 μg/ml) for overnight stimulation. Costimulatory molecule CD80 (a) and CD40 (b) expression was determined in bacteria-exposed cells cultured overnight with LPS (bold line) or without LPS (thin line). Non-exposed cells were used as positive control of LPS-induced CD40 and CD80 up-regulation. Values in the upper right corners are the mean difference (± SEM) of the percentage of cells falling in the indicated gate following overnight LPS treatment compared with unstimulated control. n ≥ 3. *P ≤ 0·05, **P ≤ 0·01, ***P≤ 0·001.