Figure 6.

Differential T-cell response to bacteria-exposed bone marrow cells. Bacteria-conditioned bone marrow cells were cocultured with ovalbumin (OVA) for 4 hr, washed extensively and then cocultured with lymph node cells from D011.10 mice at a 1 : 50 effector to responder ratio for 5 days. T-cell proliferation was determined by flow cytometric assay of bromodeoxyuridine (BrdU) incorporated during the final 6 hr of culture (a). CD4 and BrdU expression were determined on the KJ1.26⁺ lymphocytes. Cells harvested from coculture were assessed for expression of CD4 and intracellular FoxP3 by flow cytometry (b). Percentage FoxP3⁺ was determined in the CD4⁺ population. n = 3. **P ≤ 0·01, ***P≤ 0·001.