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Modulation of dendritic cell precursors by bacteria is dependent on myeloid differentiation factor 88 (MyD88). Bone marrow cells isolated from MyD88+/− and MyD88−/− mice were exposed to bacteria at D0 of culture in granulocyte–macrophage colony-stimulating factor. Flow cytometric analysis of surface markers CD11c, Gr-1 and major histocompatibilty complex class II (MHC-II) were performed following 7 days of culture. Bone marrow was pooled from six MyD88−/− and six MyD88+/− mice, n = 1.
