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. 2009 Sep;128(1 Pt 2):e325–e334. doi: 10.1111/j.1365-2567.2008.02966.x

Figure 5.

Figure 5

CCL5 induces release of nitric oxide via MEK-ERK and phosphatidyl inositol 3-kinase (PI3K) activation and disulfiram (DSF) inhibits the release of NO from RAW264.7 cells. (a) RAW264.7 cells were treated with 5 μg/ml neutralizing anti-CCR5 antibody or isotype matched immunoglobulin G (IgG). (b) Cells were treated with PD98059 (50 μm), wortmannin (100 nm) and DSF (0·1–0·3 μm) or medium with equal volume of drug solvent. 30 min later, CCL5 (50 nm) was added for the indicated times. Supernatants were collected and NO production was measured by the Griess method. Results are expressed as the mean ± SD of three independent experiments. *P< 0·05 compared with medium, †P< 0·05 compared with CCL5 and isotype-matched IgG, #P< 0·05 compared with CCL5 (50 nm) stimulation.