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Gene expression analysis of normal and mutant ovaries after gonadotropin treatment. The animals were treated for 2 days with PMSG and with hCG for 4 hr (normal, C/EBPβ+/−) and 7 hr (normal, C/EBPβ+/+), as indicated. One ovary from each animal was fixed for histological analysis (Fig. 5); total RNA was isolated from the second ovary and 10 μg was analyzed by Northern blotting with sequential hybridization to radioactively labeled cDNA probes for the indicated genes.
