Figure 2.

Effect of pharmacological inhibitors on the interferon-γ (IFN-γ)-induced nuclear translocation of protein kinase C-α (PKC-α). Adherent RAW 264.7 cells were transfected with PKC-α–green fluorescent protein (GFP) conjugate, incubated with the various pharmacological inhibitors at the concentrations indicated in the Materials and methods for 1 hr, stimulated with 100 U/ml of IFN-γ for 2 hr and then prepared for confocal microscopy analysis. At least 30 PKC-α–GFP transfected cells were counted for each inhibitor tested and the results are representative of three independent experiments. The percentage of nuclear translocation represents the ratio of nuclear PKC-α-positive cells/total transfected cells. White bars, unstimulated control; black bars, IFN-γ-stimulated cells; hatched bars, cells treated with inhibitors prior to IFN-γ stimulation.