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. 2009 Sep;128(1 Pt 2):e652–e660. doi: 10.1111/j.1365-2567.2009.03055.x

Figure 3.

Figure 3

Interferon-γ (IFN-γ)-induced phosphorylation of signal transducer and activator of transcription 1 (STAT1) on Tyr701 requires Janus activated kinase 2 (JAK2) activity. Adherent bone marrow-derived macrophages (BMDM) were incubated for 30 min in the absence or the presence of 100 or 250 μm AG-490 before the addition of 100 U/ml of IFN-γ for 15 min. Vehicle was 100% dimethyl sulphoxide (DMSO). Phosphorylation of STAT1 on Tyr701 (P-Tyr701) was determined by Western blotting, as described in the Materials and methods. Similar results were obtained in two independent experiments.