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. 2009 Sep;128(1 Pt 2):e652–e660. doi: 10.1111/j.1365-2567.2009.03055.x

Figure 6.

Figure 6

Interferon-γ (IFN-γ)-induced class II transactivator (CIITA) and major histocompatibility complex (MHC) class II (I-A) gene expression require phosphatidylinositol 3-kinase (PI3K), but not p38 mitogen-activated protein kinase (MAPK) (a and b). Bone marrow-derived macrophages (BMDM) were incubated for 1 hr in the absence or the presence of Gö-6976 (1 μm) (a), Ly294002 (100 μm), AG-490 (250 μm) or SB203580 (10 μm) (b), and stimulated for 6 hr with 100 U/ml of IFN-γ. Total RNA was extracted and subjected to reverse transcription–polymerase chain reaction (RT-PCR) using specific primers for type IV CIITA, MHC II (I-A) and Hypoxanthine-guanine phosphoribosyl transferase (HPRT) (c and d). IFN-γ-induced IFN regulatory factor-1 (IRF-1) protein expression is independent of protein kinase C (PKC), PI3K and p38 MAPK. Adherent BMDM were incubated for 1 h with or without Gö-6976 (1 μm) (c), Ly294002 (100 μm), AG-490 (250 μm) or SB203580 (10 μm) (d), and stimulated for 4 hr with IFN-γ. IRF-1 expression was assessed by Western blotting, as described in the Materials and methods. Similar results were obtained in two independent experiments.