Figure 7.

Interferon-γ (IFN-γ)-induced phosphorylation of signal transducer and activator of transcription 1 (STAT1) on serine 727 requires the activity of protein kinase C-α (PKC-α) but remains unaffected by the inhibition of phosphatidylinositol 3-kinase (PI3K) and p38 mitogen-activated protein kinase (MAPK). Adherent bone marrow-derived macrophages (BMDM) were incubated for 30 min in the absence or the presence of Ly294002 (100 μm), AG-490 (250 μm), SB203580 (10 μm), or Gö-6976 (1 μm) before the addition of 100 U/ml of IFN-γ for 30 min. Phosphorylation of STAT1 on serine 727 was determined by Western blotting, as described in the Materials and methods. Similar results were obtained in two independent experiments.