Figure 2.

Surface (a, b) and total (c, d) chemokine (C-C motif) receptor 5 (CCR5) expression in peripheral blood T4 cells from a healthy donor (a, c) and a subject with rheumatoid arthritis (RA) (b, d). Cells permeabilized (c, d) or not (a, b) were indirectly labelled with an anti-CCR5 mouse monoclonal antibody (solid line) or with a negative control antibody (dotted line) and a fluorescein isothiocyanate (FITC)-labelled goat anti-mouse immunoglobulin probe, and directly labelled with a phycoerythrin (PE)-conjugated anti-CD4 antibody. PE-positive cells were analysed for green fluorescence. A representative experiment of three is shown.