Skip to main content
. 2009 Sep;128(1 Pt 2):e442–e450. doi: 10.1111/j.1365-2567.2008.02998.x

Figure 2.

Figure 2

Bone morphogenetic protein-6 (BMP-6) induced production of inducible nitric oxide synthase (iNOS) and tumour necrosis factor (TNF)-α. The expression of BMP receptors was increased in RAW 264·7 cells. (a) BMP response element (BRE) activity was increased by BMP treatment. Cells were transfected with the reporter construct BRE-luciferase (luc). As the concentration of BMP-6 increased, the levels of luciferase activity increased (*P ≤ 0·05). Bars represent the average cell count ± standard deviation (SD). (b) Effects of BMP-6 on the expression of iNOS and TNF-α in RAW 264·7 cells. Reverse transcription–polymerase chain reaction (RT-PCR) was carried out to determine the effect of BMP-6 on the expression of iNOS and TNF-α. The expression of iNOS and TNF-α increased with BMP-6 exposure in a dose-dependent manner. (c) In RT-PCR analysis for type I and type II BMP receptors, all BMP receptors with the exception of activin receptor-like kinase 6 (ALK6) were found to be highly expressed in RAW 264·7 cells (top panel). A similar expression pattern was found in primary murine peritoneal macrophages and the human monocyte/macrophage cell line THP-1 (bottom panel).