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. 2009 Sep;128(1 Pt 2):e343–e352. doi: 10.1111/j.1365-2567.2008.02970.x

Figure 2.

Figure 2

Reversion of the T helper (Th)1/Th2 bias by bacille Calmette–Guérin (BCG) and recombinant bacille Calmette–Guérin (rBCG) in asthmatic mice. Labels indicate treatment/sensitization/challenge. D, Der p2; NS, not significant; PS, physiological saline. (a) The levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in bronchoalveolar lavage fluid (BALF) from four mice per group and the ratio of IFN-γ : IL-4 (n = 10). Data represent one of three independent experiments. (b) The levels of IFN-γ and IL-4 mRNA isolated from lung tissue were quantified using the real-time polymerase chain reaction (PCR) SYBR Green System (n = 10). The results represent the expression of the individual mRNAs with normalization to β-actin, using the cycle threshold (Ct) method. The results represent values from duplicate measurements from one of three independent experiments.