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. 2009 Sep;128(1 Pt 2):e600–e611. doi: 10.1111/j.1365-2567.2008.03041.x

Figure 2.

Figure 2

The Toll-like receptor (TLR) expression profiles in Detroit-562 and NL-20. The epithelial cell lines Detroit-562 and NL-20 were analysed for expression of TLR mRNA and proteins using real-time reverse transcription–polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis, respectively. (a, b) TLR mRNA expression in (a) Detroit-562 and (b) NL-20. The values are expressed in relation to the housekeeping gene β-actin as Inline graphic (n = 6). (c, d) Intracellular staining with monoclonal antibodies against TLRs (open histograms) or appropriate isotype controls (shaded histograms) in (c) Detroit-562 and (d) NL-20. Data show one representative out of three independent experiments.