Table 2.
Characteristics of the peptides designed for the study
| Name | Sequence | Details and use in the study |
|---|---|---|
| Bt-CLIP | Bt-PKPPKPVSKMRMATPLLMQA | Ii 82–101, reference biotinylated CLIP peptide |
| Bt-CLIP-AA | Bt-PKPPKPVSKMRMATALLMQA | Reference biotinylated CLIP-AA peptide |
| Bt-CLIP-RF | Bt-PKPPKPVSKMRMRTFLLMQA | Reference biotinylated CLIP-RF peptide |
| Bt-CLIP-QY | Bt-PKPPKPVSKMRMQTYLLMQA | Reference biotinylated CLIP-QY peptide |
| Bt-CLIP-YY | Bt-PKPPKPVSKMRMYTYLLMQA | Reference biotinylated CLIP-YY peptide |
| CLIP | SKMRMATPLLMQA | CLIP peptide |
| CLIP-AA | SKMRMATALLMQA | Mutated (P6: Pro→Ala) CLIP peptide with reduced affinity for HLA-DPGlu69 molecules |
| CLIP-RF | SKMRMRTFLLMQA | Mutated (P4: Ala→Arg; P6 Pro→Phe) CLIP peptide with higher affinity for HLA-DP DPGlu69 molecules |
| CLIP-QY | SKMRMQTYLLMQA | Mutated (P4: Ala→Gln; P6 Pro→Tyr) CLIP peptide with higher affinity for HLA-DPGlu69 molecules |
| CLIP-YY | SKMRMYTYLLMQA | Mutated (P4: Ala→Tyr; P6 Pro→Tyr) CLIP peptide with higher affinity for HLA-DPGlu69 molecules |
| TT30 | FNNFTVSFWLRVPKVSASHLE | Tetanus toxoid 947–967; HLA-DP stabilizing peptide |
Bt, biotin.