Fig. 8. Overexpression of Bdp1 or Brf1 can rescue pol III transcription in mitotic cell extracts. (A) Transcription of pLeu using 10 µg of mitotic (lanes 1–3) or asynchronous (lanes 4–6) cell extract in the presence of 1.5 (lanes 2 and 5) or 3 µl (lanes 3 and 6) of bacterially expressed recombinant Bdp1. (B) Transcription of pLeu using 10 µg of asynchronous (lanes 1–3) or mitotic (lanes 4–6) cell extract in the presence of 1.5 (lanes 2 and 5) or 3 µl (lanes 3 and 6) of bacterially expressed recombinant Brf1. (C) Transcription of pLeu using 10 µg of extract made from HeLa cells transfected with empty vector (lanes 1 and 4), 5 µg of pcDNA3HA.BRF (lanes 2 and 5) or 10 µg of pcDNA3HA.BRF (lanes 3 and 6). After transfection, the cells were synchronized in S (lanes 1–3) or M (lanes 4–6) phases prior to harvesting.