Table 1.

Characteristics of ISR-based PCR and ompW-based PCR used for identification of Vibrio cholerae

Name of primer	Target	Primer sequence (5′ → 3′)∗	Annealing†	Cycles‡	Length¶	Reference
ISR	16S–23S	AgT CAC TTA ACC ATA CAA CCC g	60–60	30	300	6
	rRNA	TTA AgC STT TTC RCT gAg AAT g
OmpW	ompW	CAC CAA gAA ggT gAC TTT AAT TgT gs	64–30	30	588	7
		gAA CTT ATA ACC ACC gCg

∗ The forward primer listed first, followed by the reverse primer

† Annealing conditions: temperature in °C—time in seconds

‡ Cycles=Number of repetitions for amplification of the dissociation, annealing, and elongation steps

¶ Length of amplification product in base pairs

ISR=Intergenic spacer region; PCR=Polymerase chain reaction