Skip to main content
. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Circ Res. 2009 Apr 9;104(9):1123–1130. doi: 10.1161/CIRCRESAHA.108.192930

Fig. 4. TRPV4 channels mediate mechanical strain induced calcium signaling in CE cells.

Fig. 4

A) Western blotting analysis showing the expression of TRPV4 in human and bovine CE cells B) Representative RT-PCR results confirming knockdown of TRPV2 and TRPV4 mRNA levels in bovine CE cells using specific siRNAs and that the same TRPV4 siRNA produced comparable suppression of protein expression (C,D; (*, p < 0.05). E) Relative change in cytosolic calcium in response to static stretch (15%, 4 sec, arrow) in Fluo-4 loaded CE cells treated with indicated siRNA. F) Average relative increases in cytosolic calcium induced by mechanical strain in CE cells treated with the indicated siRNAs (*, p < 0.02).