Fig. 6. Full-length TnsB and a TnsB peptide can provoke dissociation of a TnsCD–attTn7 complex by an ATP-dependent reaction. (A) Lanes 1 and 12 contain no Tns proteins. In lanes 2–11 TnsCD–attTn7 was incubated with TnsB protein or peptide as indicated, followed by the addition of glutaraldehyde cross-linker and separation by agarose gel electrophoresis. (B) In lane 1, no Tns proteins were added. Lanes 2 and 3 contained TnsCD–attTn7 complexes assembled in ATP, whereas lanes 4 and 5 were assembled in AMP–PNP. Lanes 3 and 5 also included the TnsB peptide.