Table I. The TnsB mutants promote increased insertion into an attTn7 + mTn7_8kb chromosome.

TnsB allele	Transposition frequency into attTn7 + mTn78kba	TnsBmut attTn7 + mTn78kbb	Transposition frequency into attTn7	Tpn. attTn7c
		TnsBwt attTn7 + mTn78kb		Tpn. attTn7 + mTn78kb
TnsABwtC + D	0.4 (± 0.13)	1d	25 (± 5.4)	62.5
TnsABP686SC + D	1.7 (± 0.50)	4.2	5.0 (± 1.2)	2.9
TnsABV689MC + D	1.9 (± 0.53)	4.8	7.8 (± 0.71)	4.1
TnsABP690LC + D	1.1 (± 0.22)	2.8	3.5 (± 0.29)	3.2

^aTransposition frequency is expressed as the ratio of chloramphenicol-resistant colonies to particle forming units of lambda phage (see Materials and methods). Each value represents the average of ≥3 trials where the final value is multiplied by 10⁴.

^bTnsAB^mutC + D transposition frequency in a strain containing attTn7 + mTn7_8kb divided by the TnsAB^WTC + D transposition frequency in a strain containing attTn7 + mTn7_8kb.

^cTnsABC + D transposition frequency in a strain containing attTn7 divided by the TnsABC + D transposition frequency in a strain containing attTn7 + mTn7_8kb.

^dThe numerator and denominator are equivalent: TnsAB^WTC + D transposition frequency in a strain containing attTn7 + mTn7_8kb.