Table 2.
Effect of diet on body weight, haem intake, concentration in faeces and daily output A: Effect of haemin and haemoglobin; B: Effect of inhibitors in a high-haemin context, and, C: in a low-haemin context.
| A | Haem in diet | No of Rats | Final body weight | Faecal output (dry weight) | Haem intake | Haem in faeces |
|---|---|---|---|---|---|---|
| Diets* | μmol/g | g/rat | g/day | μmol/day | μmol/g | |
| CD | 0 | 22 | 197 ± 9 | 0.6 ± 0.1 | 0 | 0.04 ± 0.09 |
| LH | 0.25 | 10 | 192 ± 8 | 0.5 ± 0.1 | 2.9 ± 0.1a | 0.95 ± 0.24 |
| MH | 0.5 | 10 | 187 ± 8 a′ | 0.5 ± 0.1 | 5.5 ± 0.3 a | 2.1 ± 0.7 a |
| HH | 1.5 | 20 | 178 ± 9 a | 0.8 ± 0.1a | 15.2 ± 2.2 a | 6.6 ± 1.3 a |
| HG | 1.5 | 10 | 193 ± 9 | 0.6 ± 0.1 | 16.8 ± 2.0 a | 9.6 ± 0.9 a |
| TG | 3.0 | 10 | 190 ± 10 | 0.6 ± 0.2 | 31.8 ± 3.2 a | 14.7 ± 2.3 a |
| B | ||||||
| HH | 1.5 | 20 | 178 ± 9 | 0.8 ± 0.1 | 15.2 ± 2.2 | 6.6 ± 1.3 |
| HHCA | 1.5 | 6 | 183 ± 5 | 0.8 ± 0.2 | 17.8 ± 1.4b | 7.9 ± 0.6 |
| HHAO | 1.5 | 10 | 186 ± 9 | 0.6 ± 0.1 b | 14.8 ± 1.3 | 9.5 ± 1.5 b |
| HHOO | 1.5 | 8 | 180 ± 7 | 0.6 ± 0. 1 b | 16.5 ± 0.8 | 12.3 ± 1.6 b |
| C | ||||||
| CD | 0 | 22 | 197 ± 9 | 0.6 ± 0.1 | 0 | 0.04 ± 0.09 |
| CDCA | 0 | 8 | 193 ± 5 | 1.0 ± 0.1 a | 0 | 0.01 ± 0.02 |
| CDOO | 0 | 8 | 188 ± 9 | 0.6 ± 0.1 | 0 | 0.04 ± 0.07 |
*
Dietary groups, see note to table 1.
a, a′
significantly different from control diet CD (a: p<0.01, a′: p<0.05 by Dunnett’s test)
b
significantly different from high-haemin diet HH (p<0.01, by Dunnett’s test)
c
haem metabolized was extrapolated by subtracting haem output from haem intake