Table 3.
Effect of experimental diets on the number and size of aberrant crypt foci and major aberrant crypt foci in the colon of rats after 100 days of nutritional experimentation. A: Effect of haemin and haemoglobin; B: Effect of inhibitors in a high-haemin context, and, C: in a low-haemin context.
| A | Haem in diet | No of Rats | Total Foci (ACF + MACF) | ACF c | MACF d | ||
|---|---|---|---|---|---|---|---|
| Diets* | μmol/g | Foci/colon | Size = aberrant crypts/focus | Aberrant crypts/colon | Foci/colon | Foci/colon | |
| CD | 0 | 22 | 125 ± 25 | 2.6 ± 0.2 | 324 ± 70 | 125 ± 25 | 0.3 ± 0.8 |
| LH | 0.25 | 10 | 92 ± 16a | 6.2 ± 2.0 a | 565 ± 193 a | 47 ± 23 a | 44 ± 20 a |
| MH | 0.5 | 10 | 120 ± 16 | 10.2 ± 0.9 a | 1233 ± 210 a | 12 ± 13 a | 108 ± 19 a |
| HH | 1.5 | 20 | 166 ± 26 a | 11.4 ± 1.1 a | 1901 ± 371 a | 7 ± 14 a | 158 ± 29 a |
| HG | 1.5 | 10 | 162 ± 27 a | 3.3 ± 0.4 b | 518 ± 119 b | 149 ± 23 b | 12 ± 8 b |
| TG | 3.0 | 10 | 167 ± 34 a | 2.7 ± 0.4 | 457 ± 119 b | 160 ± 32 a.b | 7 ± 6 b |
| B | |||||||
| HH | 1.5 | 20 | 165 ± 26 | 11.4 ± 1.1 | 1901 ± 371 | 7 ± 14 | 158 ± 29 |
| HHCA | 1.5 | 6 | 135 ± 22 | 2.7 ± 0.4 b | 359 ± 38 b | 131± 23 b | 4 ± 2 b |
| HHAO | 1.5 | 10 | 142 ± 25 | 2.9 ± 0.3 b | 406 ± 85 b | 135 ± 25 b | 7 ± 7 b |
| HHOO | 1.5 | 8 | 138 ± 31 | 2.8 ± 0.3 b | 389 ± 115 b | 132 ± 25 b | 7 ± 8 b |
| C | |||||||
| CD | 0 | 22 | 125 ± 25 | 2.6 ± 0.2 | 324 ± 70 | 125 ± 25 | 0.3 ± 0.8 |
| CDCA | 0 | 8 | 125 ± 11 | 2.8 ± 0.4 | 354 ± 28 | 124 ± 12 | 1.2 ± 2.3 |
| CDOO | 0 | 8 | 123 ± 18 | 2.4 ± 0.3 | 302 ± 63 | 123 ± 18 | 0.2 ± 0.7 |
*
Dietary groups, see note to table 1.
a
significantly different from control diet:CD (p<0.01, by Dunnett’s test)
b
significantly different from HH diet (p<0.01, by Dunnett’s test)
c
the median ACF contained 2.6 ± 0.2 aberrant crypts
d
the median MACF contained 11.5 ± 0.7 aberrant crypts