Figure 11.

Effects of the various nonpeptide compounds on the hV₂R-mediated MAPK activation. Stably transfected HEK293 cells with the c-myc–tagged-hV₂R were incubated for 3 min at 37°C in serum-free medium (control conditions) with either vehicle (□) or 100 nM AVP (■). Other cells were treated with 10 μM of MCF14, MCF18, MCF57, or SR121463 alone (basal; □) or in combination with 100 nM AVP (■). Then, p-ERK and total ERK from cell lysates were analyzed by Western blot. Molecular weight markers are indicated on the left of the top panel. Results are expressed as percentage of the AVP maximal p-ERK/total ERK ratio under control conditions and are the means ± SEM taken from at least three independent experiments, each performed in duplicate. A statistical analysis between control and treated cells was done: *P < 0.05.