Table 2.

Agonist properties of the nonpeptide compounds on c-myc-hV₂R–stimulated AC activity

Compound	cAMP Accumulation
	EC50 (nM)	Emax (%)
AVP	0.05 ± 0.01	100
MCF14	4.00 ± 2.00	96 ± 5
MCF18	7.00 ± 1.00	95 ± 3
MCF57	6.00 ± 2.00	103 ± 7

Accumulation of cAMP was performed as described in the Concise Methods section and supplemental information, using an homogeneous time-resolved fluorescence-fluorescence resonance energy transfer technology developed by CisBio Int. TsA201 cells transfected with the myc-hV₂R were stimulated for 30 min with increasing concentrations of AVP, MCF14, MCF18, or MCF57 in the presence of 0.1 mM of the phosphodiesterase inhibitor RO 201724. The cAMP accumulation was expressed as the mean ± SEM of cAMP concentration (nM) and reported as percentage of the maximal AVP response (Emax). EC₅₀s in the table are the mean ± SEM from three distinct experiments performed in triplicate and described in the legend to Figure 3.