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. 2009 Oct;20(10):2162–2170. doi: 10.1681/ASN.2008121232

Figure 4.

Figure 4.

Effect of SHP on the TGF-β/Smad3 signaling pathway. (A) Effects of SHP on PAI-1 promoter activity in RMCs and NRK-52E cells. Cells were cotransfected with the PAI-1 promoter and expression vectors for Smad3/4 (pRK5) and ALK5 (pcDNA). Data are the mean ± SEM of three independent measurements. *P < 0.001 compared with control, **P < 0.01, and ***P < 0.001, compared with Smad3/4 and ALK5. (B) Effect of SHP on TGF-β-stimulated Smad DNA-binding activity. Cells were incubated with TGF-β for 30 min and pretreatment of the indicated concentrations of Ad-SHP or Ad-GFP (100 moi) for 24 h.