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. 2009 Oct;20(10):2162–2170. doi: 10.1681/ASN.2008121232

Figure 6.

Figure 6.

Effect of SHP on fibrotic gene expression in the UUO model. (A) Representative RT-PCR analysis of PAI-1, type I collagen, and fibronectin expression in UUO kidneys with Ad-GFP or Ad-SHP. Rats were killed at 7 d after UUO. RT-PCR was performed on RNA from three different normal rat kidneys or UUO kidneys with or without Ad-SHP. (B) Quantification of RT-PCR results expressed as the mean ± SEM of three independent experiments (n = 9 in each group). β-actin mRNA levels were analyzed as an internal control. *P < 0.001 compared with control and **P < 0.01 compared with Ad-GFP. (C) Representative Western blot analysis of PAI-1 and fibronectin protein expression in UUO kidneys with Ad-GFP or Ad-SHP. β-actin levels were analyzed as an internal control. (D) Quantification of Western blot results expressed as the mean ± SEM of three independent experiments (n = 9 in each group). *P < 0.001 compared with control and **P < 0.01 compared with Ad-GFP.