Table 1.
Sequences of primers for RT-PCR
| Gene/Primer | Direction | Sequence (5′ to 3′) | Annealing Temperature (Cycle) |
|---|---|---|---|
| Rat | |||
| SHP | Forward | ATG-AGC-TCC-AGC-CAA-TCG-GGG-TC | 60°C (33) |
| Reverse | TCA-CCT-CAA-CAA-AAG-CAT-GTC-TTC-G | ||
| PAI-1 | Forward | AGA-TCA-GTA-CTG-CGG-ACG-CCA-TCT-TTG | 67°C (30) |
| Reverse | GCA-CGG-AGA-TGG-TGC-TAC-CAT-CAG-ACT-TGT | ||
| Mouse | |||
| SHP | Forward | CCG-CAC-CGC-ACC-TGC-AGG-GAG-GCC-TT | 70°C (25) |
| Reverse | ACT-CCA-GGC-AGC-GCT-GCA-GCC-ACT-GAA | ||
| PAI-1 | Forward | CCT-CAT-CCT-GGG-CCT-GGT-TCT-GGT-CT | 67°C (23) |
| Reverse | GGT-TTT-CCC-CGC-TGT-GGT-CAT-CTG-C | ||
| Rat and mouse | |||
| type 1 collagen | Forward | GAG-CGG-AGA-GTA-CTG-GTA-CG | 53°C (25) and 53°C (25) |
| Reverse | TAC-TCG-AAC-GGG-AAT-CCA-TC | ||
| fibronectin | Forward | ACA-CGG-TTT-CCC-ATT-ACG-CCA-T | 55°C (35) and 57°C (25) |
| Reverse | AAT-GAC-CAC-TGC-CAA-AGC-CCA-A |