Fig. 5. Effect of ectopic expression of TbSIR2RP1 and TbSIR2RP1H142Y on cell sensitivity to MMS. (A) Western blot analysis of control cells (lane 1) or cells overexpressing TbSIR2RP1 (lane 2) and TbSIR2RP1H142Y (lane 3) using anti-TbSIR2RP1 and anti-α-tubulin antibodies. (B) Subcellular localization of TbSIR2RP1 in cells overexpressing the protein. Procyclic forms overexpressing TbSIR2RP1 were subjected to IF and FISH using anti-TbSIR2RP1 antibodies and probes for telomeric repeats (Tel). Overlap of the two signals is yellow/orange. (C) Cell survival in the presence of different concentrations of MMS, comparing wild-type and cells overexpressing TbSIR2RP1 and TbSIR2RP1H142Y.