Fig. 7. Alkylated chromatin is more sensitive to MNase. Untreated and MMS-treated chromatin from wild-type procyclic cells (A) and from cell overexpressing TbSIR2RP1 (B) or TbSIR2RP1H142Y (C), or from TbSIR2RP1 knocked-down cells (D) were incubated with MNase. All digestion reactions contained 4 µg of DNA. Chromatin was digested with 0.00025, 0.0005, 0.0015 and 0.005 U of MNase for 10 min at 30°C. A negative print of the ethidium-stained DNA is shown. A 1 kb PLUS DNA ladder (Invitrogen) is on the left of each panel.