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. 2009 Oct;20(10):2171–2180. doi: 10.1681/ASN.2009030312

Figure 6.

Figure 6.

Effect of MMP9 deficiency on SCF (A and B) and c-kit (C and D) expression in E11.5 kidneys. (A) Representative SCF Western blot performed with 20 μg of total proteins from MMP9+/+ (lane 1) and MMP9−/− (lane 2) kidneys. (B) Quantitative analysis of five blots, using β-actin as an internal control, showed a 50% increase of SCF protein expression in MMP9−/− kidneys. (C) Representative Western blots detecting c-kit (lanes 1 and 2) and phospho c-kit (lanes 3 and 4) in 20 μg total proteins extracts from MMP9+/+ (lanes 1 and 3) and MMP9−/− (lanes 2 and 4) kidneys. (D) Phospho c-kit expression related to β-actin expression. Note that c-kit expression is not modified by MMP9 deficiency, whereas phospho c-kit expression is reduced by 60% in MMP9−/− kidneys. Values are mean ± SEM. ***P < 0.001 versus MMP9+/+ mice.