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. 2009 Oct;20(10):2171–2180. doi: 10.1681/ASN.2009030312

Figure 7.

Figure 7.

Sensitivity of membrane SCF to MMP9 proteolysis in vitro. (A) Representative SCF Western blot performed with concentrated medium obtained from 10 E11.5 MMP9+/+ (lane 1) or MMP9−/− (lane 2) kidneys grown for 24 h. A band corresponding to the soluble form of SCF is observed in concentrated media from MMP9+/+ kidney only. (B) ELISA of sSCF in the culture medium of E11.5 MMP9+/+ (white bar) or MMP9−/− (gray bar) kidneys grown for 24, 48, and 72 h. Values are mean ± SEM. ***P < 0.001 versus MMP9+/+ mice, n = 20.