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. 2009 Oct;20(10):2171–2180. doi: 10.1681/ASN.2009030312

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Copyright © 2009 by the American Society of Nephrology

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Figure 9. — Effect of sSCF on E11.5 MMP9^+/+ and MMP9^−/− kidney apoptosis and ureteric bud branching. (A) Percentage of apoptotic cells in MMP9^+/+ and MMP9^−/− kidneys rescued (+SCF) or not with SCF. Microphotographs of ten different kidneys sampled from MMP9^+/+ and MMP9^−/− mice rescued or not with SCF were analyzed. (B) Concentration of active caspase-3 in MMP9^+/+ and MMP9^−/− kidneys rescued or not with SCF, n = 50. (C) Number of ureteric bud tips in MMP9^+/+ and MMP9^−/− kidneys rescued (+SCF) or not with SCF. Microphotographs of ten different kidneys sampled from MMP9^+/+ and MMP9^−/− mice rescued or not with SCF were analyzed. Note that the percentage of apoptosis, the concentration of active caspase-3 activity, and the number of ureteric bud tips are not statistically different in the MMP9^+/+ kidney and in the MMP9^−/− kidney supplemented with recombinant SCF. Values are mean ± SEM. ***P < 0.001 versus MMP9^+/+-injected mice.