Table 4. Mapped EMB gene identities revealed through allelism tests and TAIL-PCR.
Locus number | Identity revealed | Mapped locusa | Mutagen | Mapped alleles | References | Cloned locus | Known allelesb | References | Identity confirmedc | Predicted protein function |
At1g08260 | Cross; PCR | EMB142* | T-DNA | 1 | [4], [5], [9] | EMB2284; EMB529; TIL1 | 6 | [21], [22] | Yes | DNA polymerase subunit |
At1g24340 | Cross | EMB260 | T-DNA | 1 | [5] | EMB2421 | 1 | No | Monooxygenase | |
At1g30610 | Cross | EMB88* (T-1002)* | T-DNA | 1 | [4], [5], [9], [19], [36] | EMB2279 | 3 | Yes | PPR protein | |
At1g55350 | Cross | EMB80 (T-98) | T-DNA | 1 | [5], [9], [19] | EMB1275; AtDEK1 | 5 | [23], [24] | Yes | Cysteine protease |
At1g63700 | Cross | EMB71 | EMS | 1 | [5], [9] | YDA | 2 | [25]-[27] | Yes | MAP3K protein kinase |
At1g79560 | Cross | EMB156; EMB36 | T-DNA; EMS | 2 | [5], [9], [18] | EMB1047 | 2 | Yes | Chloroplast FtsH-like protease | |
At2g03050 | PCR | EMB93* (T-562)* | T-DNA | 1 | [4], [5], [9], [19] | No Symbol | 0 | No | Mitochondrial transcription termination factor | |
At4g24270 | PCR | EMB140* | T-DNA | 1 | [4], [5], [9] | No Symbol | 0 | No | RNA recognition motif | |
At4g32400 | PCR | EMB104* EMB42 | T-DNA; EMS | 2 | [4], [5], [9], [18] | SHS1 | 0 | [28] | Yes | Plastid nucleotide export |
At5g02190 | Cross | EMB24 (109F-1C) | EMS | 1 | [3], [5], [9], [16], [17] | AtAsp38 | 1 | [29] | Yes | Aspartic protease |
At5g14170 | PCR | EMB262* | T-DNA | 1 | [4], [5], [9] | CHC1 | 0 | [30] | No | Chromatin remodeling |
At5g18570 | Cross | EMB269 | T-DNA | 1 | [5] | EMB3138 | 1 | Yes | GTP binding protein | |
At5g19620 | Cross | EMB213 | T-DNA | 1 | [5] | OEP80 | 2 | [31] | Yes | Chloroplast envelope protein |
At5g22800 | Cross; PCR | EMB86* EMB263 | T-DNA | 2 | [4], [5], [9], [19], [20] | EMB1030 | 2 | [32] | Yes | Alanyl tRNA synthetase |
At5g27740 | Cross; PCR | EMB161* EMB251* | T-DNA | 2 | [5], [9] | EMB2775 | 2 | Yes | Replication factor | |
At5g40160 | Cross | EMB139 | T-DNA | 1 | [5], [9] | EMB506 | 1 | [33] | Yes | Ankyrin repeat protein |
At5g41480 | Cross | EMB9 (79A) | EMS | 1 | [2], [5], [9], [15], [17] | GLA1 | 2 | [34] | Yes | Folate biosynthesis |
At5g53860 | Cross | EMB64 | X-Ray | 1 | [5], [9] | EMB2737 | 2 | Yes | Unknown | |
At5g66055 | Cross | EMB16 (123B) | EMS | 1 | [2], [5], [9], [15]-[17] | EMB2036; AKR | 2 | [33] | Yes | Ankyrin repeat protein |
At5g67570 | Cross | EMB246 | T-DNA | 1 | [5] | EMB1408; DG1 | 1 | [35] | Yes | PPR protein |
Alias symbols noted in parentheses; T-DNA tagged mutants indicated with an asterisk.
Already included at www.seedgenes.org when this project began.
Phenotype already shown to be caused by disruption of locus indicated, usually through analysis of multiple insertion alleles.