Skip to main content
. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Nat Struct Mol Biol. 2007 Nov 18;14(12):1165–1172. doi: 10.1038/nsmb1332

Figure 2.

Figure 2

YY1 associates with mammalian Ino80 complex. (a) Silver staining of the YY1 complex separated on 4%–12% SDS-PAGE gel. The YY1 complex was immunopurified from nuclear extract prepared from HeLaS cells stably expressing Flag-HA-YY1. Subunits of INO80 complex are labeled in gray. (b) The double-purified YY1 complex in a was further fractionated by 10%–40% glycerol-gradient sedimentation. Fractions were visualized by silver staining (top) and immunoblotting with indicated antibodies (bottom). Input (3%) and molecular weight (MW) marker were also included on the silver-staining gel. (c) YY1 interacts with subunits of INO80 complex in vivo. Total HeLa cell lysates were immunoprecipitated with BAF53, TIP49A and TIP49B antibodies. YY1 levels in immunoprecipitates and 15% input were compared by western blotting with the YY1 antibody.