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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Methods. 2009 May 4;48(4):346–352. doi: 10.1016/j.ymeth.2009.02.026

Figure 2. Influences of immidazol on transcription of isolated elongation complexes.

Figure 2

Isolated EECs were allowed to elongate transcripts for six minutes in the absence or presence of indicated concentrations of immidazol and/or purified factors (0.45 pmol of DSIF and 0.03 pmol of NELF). The synthesized transcripts were extracted and analyzed on a 6% denaturing RNA gel followed by autoradiography of the dried gel.