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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Methods. 2009 May 4;48(4):346–352. doi: 10.1016/j.ymeth.2009.02.026

Figure 5. Examination of EC•factor interaction using EC-EMSA.

Figure 5

A. Association of TFIIF with ECs. Isolated EECs were released from the beads by SacI digestion (crestriction site is 17 bp upstream of transcription start site). Then the complexes were left untreated or incubated with indicated amounts of TFIIF before analysis on a native gel. B. Nascent RNA facilitates the EC•DSIF interaction. Isolated EECs were left untreated or further elongated for three minutes before being released by SacI. Then the elongation complexes were incubated without or with 0.3 pmol of DSIF and further analyzed on a native gel.