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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Mol Cell Proteomics. 2006 May 31;5(8):1514–1519. doi: 10.1074/mcp.T600013-MCP200

Fig. 2. Reactivity of purified E. coli O157 LPS and recombinant E. coli BL21(DE3) (pET-30b) expressing various bacterial proteins with pooled hyperimmune sera from cattle experimentally infected with E. coli O157.

Fig. 2

a, dot immunoblot of purified O157 lipopolysaccharide (LPS) spotted in duplicate and screened with pooled unadsorbed hyperimmune bovine sera. b, 1, dot immunoblot of O157 lipopolysaccharide spotted in duplicate (test); 2, colony immunoblot of four recombinant clones expressing PilA of Vibrio cholerae, an irrelevant protein (control); and 3, colony immunoblot of four recombinant clones expressing EspB of E. coli O157 (test) screened with pooled hyperimmune bovine sera adsorbed against purified E. coli O157 lipopolysaccharide.