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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Mol Cell Proteomics. 2006 May 31;5(8):1514–1519. doi: 10.1074/mcp.T600013-MCP200

Fig. 3. SDS-PAGE profiles of recombinant O157 proteins in elutions from columns coupled to bait pAbs (charged) or uncharged columns.

Fig. 3

Multiple O157 expression library cultures were induced with a range of IPTG concentrations at an initial A600 of 0.6. Following incubations at different temperatures for varying lengths of time, cell lysates and pellets prepared from pooled, pelleted cultures were loaded on charged or uncharged columns. Elutions were fractionated on SDS-PAGE Tris-Glycine 8−16% gradient gels and visualized by SimplyBlue Safe Stain. a, elutions from a charged column (test) loaded with lysate fractions. b, elutions from an uncharged column (control) loaded with lysate fractions. c, elutions from a charged column (test) loaded with pellet fractions. d, elutions from an uncharged column (control) loaded with pellet fractions. l, molecular weight ladder.