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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: J Biomol Screen. 2008 Sep 23;13(9):870–878. doi: 10.1177/1087057108323909

Figure 3. Determination of EC50s of known calpain inhibitors.

Figure 3

Figure 3

EC50 values for MDL-28170 (A) and ALLN (B) were determined by treating SH-SY5Y cells with inhibitor for 30 minutes, followed by incubation with Suc-LLVY-aminoluciferin substrate for 30 minutes. SH-SY5Y cells were lysed in calpain lysis buffer and incubated with detection reagent for 15 minutes, and luminescence measured. Error bars represent the standard deviation among 8 replicate wells, and data are representative of the results of 2 separate experiments.