Skip to main content
. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: Curr Biol. 2009 Apr 2;19(8):688–693. doi: 10.1016/j.cub.2009.02.055

Figure 4. Mitotic clones of Cdk9 and Elo-A display patterning phenotypes.

Figure 4

The mitotic clones of Cdk9 and Elo-A was generated using standard techniques and was screened for Ubx phenotype (haltere to wing transformation) in the adult flies. Mitotic clones of Cdk9 (B) and Elo-A (C) in adult haltere lead to transformation of haltere to wings (as seen by emergence of wing-like bristles) that is absent in wt haltere (A). Haltere imaginal discs containing Cdk9 clones [seen by using GFP as marker. Note the Cdk9−/− clone marked by no GFP and twin spot marked by GFP+/+ in a background of GFP+/−. (D)] lack Ubx expression [seen by anti-UBX staining in red. Note the loss of UBX staining from the Cdk9−/minus; clone and normal staining of Ubx in GFP+/+ clones (E)]. DAPI (F) was used to mark cell nuclei in the haltere imaginal discs. The adult mitotic clones of Cdk9 and Elo-A display phenotypes associated with signaling mutants. Cdk9 clones show ectopic wing veins (H), notched wings (I). Elo-A mitotic clones display similar signaling phenotypes of ectopic wing veins (J), short wing cross-veins (K), duplication of macrochaete in adult notum (L, shown by arrow) and loss of macrochaete from notum region (M, shown by dashed circles). All these phenotypes suggest perturbations in Notch, EGF, and Dpp (TGFβ) signaling