Fig. 2. Molecular characterization of the spnA alleles and DmRad51 protein expression analysis of mutant ovary extracts. (A) Map of the DmRad51 mutations. Amino acid substitution and allele name are given (in parentheses). (B) Western blots of extracts prepared from wild-type and hemizygote mutant ovaries. Walker-A and -B box motifs are indicated by black rectangles. Both the wild-type (wt; Oregon R) and heterozygote line, spnA^093A/TM3, show expression of a 36.6 kDa protein (arrow). Band shift (arrowhead) in spnA^048B/Df(3R)X3F ovary extracts confirms the specificity of the antibody. The lower running band (double arrowhead) is a non-specific cross-reactive protein.