Fig. 7. Persistence of γ-HIS2AV staining suggests a defect in the repair of meiotically induced DSBs. (A and B) Confocal images of different oogenesis stages from a wild-type (spnA093A/TM3) ovary. (C and D) Confocal images of similar oogenesis stages from a spnA [spnA093A/Df(3R)X3F] ovary. DNA is stained with OliGreen® (green). Early cysts and the oocytes are marked by immunostaining for Orb protein (blue). (A and A′) γ-HIS2AV speckles the DNA in one cell (A′, red, arrow), presumably the oocyte. A 2× zoom of that nucleus is provided in the A′ inset. (C and C′) γ-HIS2AV observed in the germarium of spnA ovaries. (C′, arrows) Two cells of a common cyst (D) γ-HIS2AV in spnA mutants beyond the germarium in the vitellogenic stages of oogenesis. All images are single confocal sections.