Table 2. Comparison of ΔG^0’ from literature and ΔG⁰_ec calculated from metabolite concentrations.

All calculations assume a concentration of 20 mM inorganic phosphate⁴⁷, and that intracellular pH is the same (for literature values, pH 7) in all three culture conditions. All values are in kJ/mole.

		from concentrations		Literature		Overlap
From	To	min ΔG0ec(kJ/mol)	max ΔG0ec(kJ/mol)	min ΔG0’(kJ/mol)	max ΔG0’(kJ/mol)	Min	Max
F6P + ATP	FBP + ADP	N/Aa	9.3	−22.1	−14.2	−22.1	−14.2
FBP	F6P + Pi	N/Aa	−2.2	−14.1	−10.0	−14.1	−10.0
FBP	DHAPb	21.5	29.3	10.3	23.1	21.5	23.1
DHAP + NAD + ADP + Pi	3PG + NADH +ATP	−22.9c	−40.1c	−12.6	−0.30	-	-
3PG	PEP	−2.32	4.3	−1.0	8.0	−1	4.3

^aN/A: There is no lower bound on ΔG⁰_ec using concentrations, as the reactions only need to work in one direction.

^bkJ/mole for FBP and kJ/(2 mole) for DHAP

^cA possible explanation for the requirement for a large negative ΔG⁰_ec value for this reaction is mismeasurement of one or more reactants or products. A particular possibility is that 1,3-diphosphoglycerate is hydrolyzed to 3PG during metabolome extraction, leading to erroneously high measured 3PG concentration.