Fig. 7. Mss51p interacts with newly synthezised Cox1p. Translation in mitochondria isolated from MSS51 (WT) or MSS51-HA (HA) strains was performed at 25°C in the presence of [³⁵S]methionine. Samples were removed at the indicated labeling times (pulse). A fourth sample was pulse-labeled for 30 min and then chased by addition of 10 mM cold methionine followed by incubation at 25°C for 30 min (chase). Mitochondria were washed and solubilized, and the solubilized products were immunoprecipitated with anti-HA antibody (α-HA). Translation products were analyzed by SDS–PAGE and autoradiography. The total aliquots represent 10% of the aliquots used for immunoprecipiation. Abbreviations are as in Figure 5C; ATPase subunit 8, Atp8; subunit 9, Atp9. Strain details are given in the Supplementary data.